The work was carried out following 241 and 23 experimental designs in the presence and absence of inducers, respectively, aiming to evaluate the influence of some variables in the lipase production. Aspergillus oryzae an overview sciencedirect topics. A list of various fungal lipase producers is presented in table 1. Lipase production in aspergillus niger j1 was tested using both submerged fermenta tion smf and solidstate fermentation ssf on a mineral culture medium and wheat bran, respectively. Solid state fermentation of lipase p roduction from. In the present work, to facilitate bulk synthesis, lipase production using aspergillus niger mtcc 872 was studied by solidstate fermentation ssf. Production of extracellular lipase from aspergillus niger by. The extracellular lipase producing fungus was isolated from spent bleaching earths. Lipase production in aspergillus niger j1 was tested using both submerged fermentation smf and solidstate fermentation ssf on a mineral culture medium and wheat bran, respectively. Case report biodegradation activity of aspergillus niger. Screening, optimization and process scale up for pilot.
Aspergillus species, lastly modified lipase medium was prepared to make suspension and mass culturing of the aspergillus niger fungi, it involved addition of 10. Lipase is one of the most used industrial enzymes, which can be produced by aspergillus niger. Pdf production of lipase immobilized cells of aspergillus niger. Nitrous acid mutants exhibited increased efficiency for lipase production when compared with uv mutants in submerged fermentation. In this study, aspergillus niger adm110 fungi was gamma irradiated to produce lipase enzyme and then immobilized onto magnetic barium ferrite nanoparticles bfn for biodiesel production. The hyperproducing uv and nitrous acid mutants were further subjected to a.
Cultural conditions for the production of lipase by aspergillus niger strain mtcc 2594 by solidstate fermentation using gingelly oil cake were standardized. Bala 1 departmentofbiochemistry,ahmadubellouniversity,zaria,nigeria department of biochemistry, bayero university, kano, nigeria. The isolation and purification of lipolytic activities were performed by. Gomashe and shrungi parlewar department of microbiology, shri shivaji science college, congress nagar, nagpur, india corresponding author abstract introduction lipases are wellknown as one of the important group of enzymes in international. Research article application of plackettburman experimental design for lipase production by aspergillus niger using shea butter cake aliyusalihu, 1 muntaribala, 2 andshuaibum. Pdf studies on the lipase production by aspergillus niger through. Corn steep liquor as a nutrition adjunct for the production of aspergillus niger lipase and hydrolysis of oils thereof. Aspergillus niger ncim 1207 produces extracellular and mycelium bound lipase which is. With the aim ofintroducing the application of lipase in the palm oil industry, we have isolated aspergillus niger sbs k020 as a potential producer of exogenous lipase. Myceliumbound lipase production from aspergillus niger mya. Characterization of different oil soapstocks and their application in the lipase production by aspergillus niger under solid state fermentation. Production of extracellular protease enzyme by aspergillus. Lipase production by aspergillus niger c by submerged.
Lipase produced by aspergillus niger is widely used in various industries. A 276% increase in lipase production was achieved by strain improvement of a. Abstractthe effects of medium components affecting lipase production by aspergillus niger as02 using sheanut cake snc were studied based on the onefactoratatime ofat method and the facecentred central composite design fcccd. Many industrial lipases have been produced, purified and cloned from bacteria and fungi that include aspergillus species. The food enzyme is free from viable cells of the production organism and recombinant dna. The chosen fungal strain was evaluated for lipase production using a mixture of agroindustrial substrates viz. It was identified as aspergillus niger by macro and micromorphological analysis sidrim and rocha, 2004. Screening, optimization and process scale up for pilot scale production of lipase by aspergillus niger. Elitol and ozer 30 immobilized the whole cell of r. Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel. Production and optimization of lipase using aspergillus. The lipase had a molecular weight, km and v max about 19 kda, 0.
A lipase activity of 3636 ug of dry substrate was obtained at 72 h under optimum conditions. Each was cultivated on four different media differing in their contents of mineral components and sources of carbon and nitrogen. Statistical optimization by response surface methodology. Lipases are ubiquitous enzymes of considerable physiological significance and industrial potential.
In this work the lipase enzyme production by the mutant aspergillus niger 11t51a14, which is a wellknown lipase producer was studied using wheat bran as substrate. Lipase production from aspergillus niger, by solidstate fermentation using gingelly oil cake. Issn 309862 original scientific paper ftb1670 production of extracellular lipase from aspergillus niger by solidstate fermentation gwen falony1, janny coca armas1, julio c. Pdf production of acidic lipase by aspergillus niger in. Out of the eleven medium components screened, six comprising of sucrose, nh 4 2 so 4, na 2 hpo 4, mgso 4, tween80, and olive oil were found to. In our study, we compared both batch and fedbatch process to determine their efficiency in scale up production of alkaline lipase from aspergillus niger mtcc 2594 using submerged stir tank fermenter sstf. The optimization of the culture medium was carried out for both.
Screening and identification of a novel lipase producing. Statistical optimization by response surface methodology to. Research article optimization of fermentation medium for. Lipases are used industrially for the resolution of chiral compounds and the transesterification production. Characterization of lipase from wild lpf5 and mutant hn1. Production and partial characterisation of extracellular lipase from aspergillus niger. Use of goc along with wb as potential raw materials for enzyme production could be of great commercial significance. The food enzyme triacylglycerol lipase triacylglycerol acylhydrolase, ec 3. Enhanced production of extracellular lipase by novel mutant. Biodiesel production by aspergillus niger lipase immobilized. View the article pdf and any associated supplements and figures for a period of 48 hours. Elkhawaga 3,d 1drug radiation research department, national center for radiation research and technology ncrrt, atomic energy authority, egypt.
Production and stabilization of amylases from aspergillus niger. Based on these results, we conclude that jatropha seed cake is a suitable medium for lipase production. The influence of various oil cakes has been investigated for high level production of lipase using aspergillus tamarii mtcc 5152. Regiane ribeiro dos santos 1, livia nolasco macedo muruci 1, lucielen oliveira santos 1, rosemar antoniassi 2, janine passos lima da silva 2, monica caramez triches damaso 3. Forty each of aspergilli and penicillia were screened for extracellular lipase production on agar plates and in liquid medium containing olive oil as substrate. Isolation, purification, and characterization of lipase. Pdf lipase production by aspergillus niger c by submerged. Bfn were prepared by the citrate solgel autocombustion method and characterized by transmission electron microscopy tem, xray diffraction xrd, fourier transform infrared ftir and scanning electron.
Enzyme production was considered high under room temperature after 4 days using sunflower oil and a combination of casein with sodium nitrate. Pdf biodiesel production by aspergillus niger lipase. Production of extracellular lipase from aspergillus niger. Mixed substrate solid state fermentation for production and. Rai, baplu, ashish shrestha, shishir sharma, and jarina joshi. Using fermentation processes for production of lipase by aspergilluse niger afshin. Aspergillus fumigatus and aspergillus nidulans produce toxins, so they are not used for the production of recombinant enzymes.
Fifty strains of species of rhizopus, mucor, geotrichum, penicillium and aspergillus were screened for their ability to form butyl esters from butter oil in the presence of butanol. Pdf production of extracellular lipase from aspergillus. Pdf the current study includes isolation and screening of different fungal cultures from soil samples for lipase production by solid state. Enhanced production of extracellular lipase by novel. Lipase production by aspergillus niger 11t53a14 in wheat bran. Further studies were performed for scaleup of lipase from flask level to lab scale using tray fermenter. Production and optimization of lipase by aspergillus niger using. Aspergillus niger, solidstate fermentation, lipases. The enhancement of lipase production from aspergillus niger was attempted by ultraviolet uv and nitrous acid mutagenesis, and the mutants were selected on media containing bile salts. Lipase production from aspergillus niger by solidstate. A 200% increase in lipase yield by aspergillus niger mutant from uv and ntg treatments ellaiah et al, 2002.
Aspergillus niger to produce lipase and obtained similar activities for both free and immobilized biomass cultivations 4 u ml. Pdf strain improvement of aspergillus niger for the. This is the first report on the production of lipase from aspergillus niger mtcc 872 using trisubstrate mixture. Myceliumbound lipase production from aspergillus niger mya 5, and its potential applications for the transesterification of ethanol. Pdf characterization of aspergillus niger 65i6 lipase. Lipases from aspergillus niger are produced both intracellularly and extracellularly 54, 55. Optimization of fermentation medium for extracellular lipase. Lipolytic potential of aspergillus japonicus lab01. Abstract the present investigation was carried out to evaluate the effect of different growth conditions on lipase production by aspegillus niger. Response surface analysis for the production of an enantioselective lipase from aspergillus niger by solidstate fermentation. Lipase production by aspergillus niger using sheanut cake.
Production of lipase by immobilized cells of aspergillus niger. Optimization of physical and chemical parameters was done for maximum lipase production using this isolate. An indigenous strain of aspergillus niger au15 which produced lipase constitutively was used to produce mutants using ultraviolet and nmethylnnitronnitosoguanidine. Production of lipase from aspergillus niger wild lpf5 and hyperproducer nitrous acid mutant strain hn1 of a. Effect of bio process variables such as ph, temperature and substrate concentration of lipase activity were also monitored during lipase production. Microorganism used in fermentation process was inoculated at a concentration of 10 ap6 cas number 9001621. Four strains of aspergillus niger were screened for lipase production. Research article application of plackettburman experimental. Ataie salehi p 3 abstractthe lipase isan extracellular enzyme in food industry and is produced by different microbial and fungal sources especially aspergilluse niger aspergillus nigerby submerged fermentation and solid state fermentation processes. Pdf lipases have various industrial applications and their production by filamentous fungi in solid state fermentation ssf process is of interest find, read.
Pdf lipase production by aspergillus niger 11t53a14 in wheat. Screening, optimization and process scale up for pilot scale production of. Purification and characterization of a lipase from. Ataie salehi p 3 abstractthe lipase isan extracellular enzyme in food industry and is produced by different microbial and fungal sources especially aspergilluse niger aspergillus nigerby submerged. Spore suspensions were prepared from 7 days old slant culture. Abstracttwelve fungal strains belonging to 9 different species of genera aspergillus, pencilillium, trichoderma and mucor were screened for extracellular lipase production.
Species of the mold aspergillus are well known lipase producers. A qualitative screening revealed the occurrence of lipase, esterase, protease, amylase, endo. Lipase production from aspergillus niger by solidstate fermentation. The present study was carried out to determine the production of the lipase enzyme from aspergillus flavus under submerged fermentation. Application of plackettburman experimental design for lipase. Lipase production by aspergillus and penicillium species. Characterization of aspergillus niger 65i6 lipase from. Aspergillus niger, or black mold, is a common fungus that appears on decomposing starchy fruits and vegetables as well as on damp walls as a component of mildew. The secondary carbon source, nitrogen source, and lipid were found to be the three most influential factors for lipase production by singlefactor experiments.
Optimization of fermentation medium for extracellular. Lipase enzyme production will be tested on rhodamine b agar medium 9. Production and optimization of lipase using aspergillus niger mtcc 872. Optimization of aspergillus niger lipase production has been performed in this study. This organism plays an important role in food science, including the production of citric acid, which serves as a preservative and flavoring agent for soft drinks like soda. Different effects in lipase production have been reported depending on the nitrogen source and the microorganism 3. Production of extracellular lipase from aspergillus niger by solid.
Production of acidic lipase by aspergillus niger in solid. Characterization of different oil soapstocks and their. Recently, lipase catalyzed production of various esters such as isoamyl butyrate, isoamyl propionate, hexyl butyrate and laurate, 14, citronellyl flavor ester has been investigated. V the genetic modifications do not give rise to safety concerns. Effect of environmental and nutritional parameters on the. Production of lipase enzyme by aspergillus flavususing. Enhanced production of aspergillus tamarii lipase for. By solid state fermentation in wheat bran containing 2. Aspergillus oryzae and aspergillus niger are on the generally recognized as safe gras list of the food and drug administration fda in the united states. Using fermentation processes for production of lipase by. In other studies, 72 h was reported as the optimal fermentation time for aspergillus niger lipase grown in solidstate fermentation, with a subsequent decrease in activity seen at 96 h and 120 h 22 kamini nr, mala jgs, puvanakrishna p. Communication i partial glycerides synthesis by lipase of. Lipase production in aspergillus niger j1 was tested using both submerged fermenta tion smf and.
Purification and characterization of a lipase from aspergillus niger f044 article in sheng wu gong cheng xue bao chinese journal of biotechnology 231. Plackettburman design was used to efficiently select important medium components affecting the lipase production by aspergillus niger using shea butter cake as the main substrate. A novel mixed substrate solidstate fermentation ssf process has been developed for aspergillus niger mtcc 2594 using wheat bran wb and gingelly oil cake goc and the results showed that addition of goc to wb wb. Mixed substrate solid state fermentation for production. Pdf strain improvement of aspergillus niger for enhanced. Lipase production by aspergillus niger 11t53a14 in wheat. Ammonium salts supported the best growth as well as lipase production by aspergillus niger 11. This is the first report on the production and extraction of lipase from aspergillus niger using mixed solid substrates, wb and goc, which are potential raw materials for the production of enzymes and other valueadded products. Lipases are ubiquitous enzymes of considerable physiological significance and. Lipase production by aspergillus niger c by submerged fermentation article pdf available in brazilian archives of biology and technology 623 january 2019 with 31 reads how we measure reads. Lipase production by aspergillus niger grown in different.
Characterization of aspergillus niger 65i6 lipase from solidstate fermentation using jatropha seed cake medium article pdf available april 2017 with 201 reads how we measure reads. Lipases have various industrial applications and their production by filamentous fungi in solid state fermentation ssf process is of interest. Large scale production of aspergillus niger lipase is more profitable by using solid state fermentation method and utilizing agroindustrial waste as the substrate. Optimization of aspergillus niger lipase production by. Safety evaluation of the food enzyme triacylglycerol. Lipase production by aspergillus niger c by submerged fermentation. Lipase production from aspergillus niger by solid state fermentation using gingelly oil cake. Lipolytic potential of aspergillus japonicus lab01 was investigated by describing the catalytic properties and stability of a secreted extracellular lipase. The results also suggested that the produced lipase from aspergillus niger 6516 was an alkaline lipase.
Effect of environmental and nutritional parameters on the extracellular iipmroduction by aspergillus niger ahmed i. Production of lipase by immobilized cells of aspergillus niger vaidehi chandorkar, a. In this study, extracellular lipase production from an industrial producing strain of a. Aspergillus niger is a fungus and one of the most common species of the genus aspergillus it causes a disease called black mold on certain fruits and vegetables such as grapes, apricots, onions, and peanuts, and is a common contaminant of food. Production and optimization of lipase using aspergillus niger mtcc. Among the various fungal strains screened for lipase production, rhizopus arrhizus ncim 877, 878, 879 and aspergillus niger ncim 1207 produced significant quantities of enzyme when grown in synthetic oil based sob medium under submerged conditions. Lipase production from aspergillus niger, by solidstate fermentation.
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